pyromark therascreen mgmt kit Search Results


luciferase assay system kit  (Promega)
90
Promega luciferase assay system kit
Luciferase Assay System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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optocomp1 luminometer  (Mgm Instruments)
90
Mgm Instruments optocomp1 luminometer
Optocomp1 Luminometer, supplied by Mgm Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genemaptm mgmt methylation analysis kit  (GenMark Diagnostics)
90
GenMark Diagnostics genemaptm mgmt methylation analysis kit
Genemaptm Mgmt Methylation Analysis Kit, supplied by GenMark Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pyromarktm mgmt kit  (BIOTAGE)
90
BIOTAGE pyromarktm mgmt kit
Pyromarktm Mgmt Kit, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mgm csf elisa kit  (OriGene)
90
OriGene mgm csf elisa kit
Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by <t>ELISA;</t> n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.
Mgm Csf Elisa Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mgmt methylation detection kit  (EntroGen Inc)
90
EntroGen Inc mgmt methylation detection kit
Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by <t>ELISA;</t> n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.
Mgmt Methylation Detection Kit, supplied by EntroGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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mgmt plus kit  (Pyrosequencing Inc)
86
Pyrosequencing Inc mgmt plus kit
Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by <t>ELISA;</t> n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.
Mgmt Plus Kit, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mgm csf quantikine elisa kit  (R&D Systems)
94
R&D Systems mgm csf quantikine elisa kit
Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by <t>ELISA;</t> n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.
Mgm Csf Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qiamp dna ffpe tissue kit  (Qiagen)
95
Qiagen qiamp dna ffpe tissue kit
Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by <t>ELISA;</t> n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.
Qiamp Dna Ffpe Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mgm csf  (Elabscience Biotechnology)
94
Elabscience Biotechnology mgm csf
Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by <t>ELISA;</t> n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.
Mgm Csf, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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digene dcr-1 luminometer  (Mgm Instruments)
90
Mgm Instruments digene dcr-1 luminometer
Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by <t>ELISA;</t> n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.
Digene Dcr 1 Luminometer, supplied by Mgm Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by ELISA; n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.

Journal: The Journal of experimental medicine

Article Title: Group 2 innate lymphoid cells support hematopoietic recovery under stress conditions.

doi: 10.1084/jem.20200817

Figure Lengend Snippet: Figure 1. HSPCs in stressed BM receive strong GM-CSF signals. (A–C) LSK cells, which were sorted from CAG-EGFP mice, were transplanted into steady- state control (Ctrl) or 5-FU–treated WT mice. Then, EGFP+ cells in the recipient BM were sorted from two groups, and RNA-seq analyses were conducted. (A) Schematic of the procedure. (B) Flow cytometry plots of BMMNCs of untreated (control) or 5-FU–treated recipient WT mice that were transplanted with LSK cells from CAG-EGFP mice. Results shown are representative of two independent experiments. (C) Heat map of differentially expressed genes in RNA-seq analyses of HSPCs transplanted and homed into recipient BM (5-FU versus control); n = 2 per group. (D–F) Phenotypical changes of WT mice through a single 5-FU (200 mg/kg) injection. Each experiment was performed on day 0 (control) and day 2. (D) qRT-PCR analyses for the expression of indicated genes of total BM cells and ECs; n = 3, representative of two independent experiments. (E) The numbers of total BM cells in femurs and tibias; n = 3, representative of two independent experiments. (F) GM-CSF protein levels in femur analyzed by ELISA; n = 3, representative of two independent experiments. (G) qRT-PCR analyses for the expression of Csf2ra and Csf2rb genes in Lin−cells, CAR cells, and ECs that were collected from BM of WT mice 2 d following 200 mg/kg 5-FU injection; n = 3 or 4, representative of two independent experiments. In the bar charts, the results are shown as mean ± SEM, and each dot represents an individual mouse. Statistical significance was determined by unpaired Student’s t test. ND, not detectable.

Article Snippet: GMCSF and IL-33 were measured using the mGM-CSF ELISA Kit (Origene) and mouse/rat IL-33 Quantikine ELISA Kit (R&D Systems), according to the manufacturer’s instructions.

Techniques: Control, RNA Sequencing, Flow Cytometry, Injection, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay